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Whetstone et al., Vet Microbiol, 16: 15 (1988) [PMID: 3281371] CABRI:DSMZ_MUTZ Accession_number ACC 178 Cell_line_name 2E10-H2 Brief_description confirmed as mouse with IEF of AST, NP established by fusion of SP2/0-AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 1 (CAV-1); the secreted antibody binds to and neutralizes CAV-1 and is type-specific Description mouse hybridoma (anti-canine adenovirus type 1) established by fusion of SP2/0- AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 1 (CAV-1); the secreted antibody binds to and neutralizes CAV-1 and is type-specific confirmed as mouse with IEF of AST, NP Depositor Dr. H. G. Drexler, DSMZ, Braunschweig, Germany Morphology single cells in suspension Culture_conditions 92.5% Dulbecco's MEM (4.5 g/L glucose) + 5% horse serum + 2.5% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days at 37C with 5% CO2 maximum density at about 1-2 x 106 cells/ml Viruses ELISA: reverse transcriptase negative; PCR: SMRV - Properties Release_conditions - Hazard - Storage frozen with 70% medium, 10% horse serum, 5% FBS, 10% DMSO at about 5 x 106 cells/ampoule Mycolasma negative in DAPI, microbiological culture, RNA hybridization assays Cytogenetics 92(88-101)<4n> with 1.7% polyploidy - predominantly mouse chromosome morphology with 3 submetacentric marker chromosomes Bibliographic_reference Whetstone et al., Intervirology, 23: 15 (1985) [PMID: 3884549 ]

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